Journal: Journal of Translational Autoimmunity

Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

doi: 10.1016/j.jtauto.2026.100359

Figure Lengend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

Article Snippet: The reactivity of the patients’ sera and/or CSF against the retina was evaluated retrospectively with frozen samples (−80°, EXPLAINEUR biobank) through an indirect immunofluorescence technique using sections of monkey retina (Ref. FA1172-1005, Euroimmun), detected with an FITC-labelled secondary antibody anti-human IgAGM (Euroimmun conjugate) or directed against IgA (ref. F0204, DAKO), IgM (ref. F0203, DAKO), IgG1 (ref. 9052-02, Southern Biotech) and IgG4 (ref. 9200-02, Southern Biotech).

Techniques: Immunofluorescence, Fluorescence, Control

Journal: Journal of Translational Autoimmunity

Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

doi: 10.1016/j.jtauto.2026.100359

Figure Lengend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

Article Snippet: The reactivity of the patients’ sera and/or CSF against the retina was evaluated retrospectively with frozen samples (−80°, EXPLAINEUR biobank) through an indirect immunofluorescence technique using sections of monkey retina (Ref. FA1172-1005, Euroimmun), detected with an FITC-labelled secondary antibody anti-human IgAGM (Euroimmun conjugate) or directed against IgA (ref. F0204, DAKO), IgM (ref. F0203, DAKO), IgG1 (ref. 9052-02, Southern Biotech) and IgG4 (ref. 9200-02, Southern Biotech).

Techniques: Immunofluorescence, Fluorescence, Control

Journal: Journal of Human Immunity

Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome

doi: 10.70962/jhi.20250119

Figure Lengend Snippet: B cell−extrinsic elevation of circulating plasmablasts and increase in IgE isotype switching in the patient. (A) Flow plots of main B cell subsets (top row) and antigen-experienced B cell subsets (bottom row) in one healthy control (left panels) and the patient (right panels). Representative of two independent analyses. (Data shown in rows 1 and 2, column 1 are identical to data in , row 1, column 4 and 5.) (B) Gate frequencies for antigen-experienced B cell subsets of five healthy controls (HCs) and the patient. Representative of two independent analyses. (C) Fraction of IgE class-switched cells among antigen-experienced B cell subsets of three healthy controls and the patient. The individual points for healthy control and patient represent one each of three sampling methods (conventional Ficoll prep, separator tubes, and whole-blood RBC lysis) from one experiment. (D) Schematic overview of iGB culture setup (created with BioRender). (E) Cell counts for one representative of two similar experiments. (F) IgM, IgG1, IgG3, and IgE secretion in cultures, normalized to cell count. Full gating strategies are shown in . Statistics were calculated using the unpaired t test. * = P < 0.05. aMBC_PC, aMBC/plasma cell; DN, double-negative; MBC_only, conventional memory B cell; SWmem, switched memory; T1, T2, T3, transitional 1, 2, 3 subsets.

Article Snippet: Wells of FluoroNunc MaxiSorp 96-well plates were coated with 100 μl capture antibody diluted in PBS, either 0.5 μg/ml goat α-human IgM-Hc (2023-01; Southern Biotech), 5 μg/ml mouse α-human IgG1-Fc (9054-01; Southern Biotech), 1 μg/ml mouse α-human IgG3-hinge (9210-01; Southern Biotech), or 2.5 μg/ml mouse α-human IgE-Fc (9240-01; Southern Biotech).

Techniques: Control, Sampling, Red Blood Cell Lysis, Cell Characterization, Clinical Proteomics